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December 10, 2020
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Glycine additive facilitates site-specific glycosylation profiling of biopharmaceuticals by ion-pairing hydrophilic interaction chromatography mass spectrometry
Many biotherapeutics corresponding to monoclonal antibodies (mAb) and Fc-domain fusion proteins include heterogeneous glycan contents at one or a number of glycosylation website(s). Website-specific glycan profile characterization is important for monitoring the standard of those molecules throughout totally different phases of drug improvement. Hydrophilic interplay chromatography (HILIC) as an orthogonal separation methodology to reversed-phase liquid […]
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