Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic 

Magainin Pharmaceuticals Inc. – Biopharmaceutical Research  > Biology Cells, Clia Kits, Enzymes, Equipments, Pcr Kits >  Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic 
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Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

 

Background: Propofol is a standard sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been absolutely elucidated.

 

Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Equipment-8 (CCK-8) to detect proliferation. Apoptosis was measured with move cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or detrimental management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 

small interfering ribonucleic acid (siRNA) or detrimental management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression have been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR). In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice have been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors have been then collected from mice and analyzed by immunohistochemistry and Western blotting.

 

Outcomes: Propofol inhibited development (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by reducing PTEN protein

 

expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol remedy lowered NSCLC tumor development (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

 

Conclusions: Our examine indicated that propofol inhibited A549 cell development, accelerated apoptosis through the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell development, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical remedy.

 

RFP (mRuby3) Control Lentivirus

LV101 4 x 500 ul
EUR 195

RFP (TagRFP-T) Control Lentivirus

LV097 4 x 500 ul
EUR 195

Negative Control Luciferase Lentivirus

79578 500 µl x 2
EUR 860
Description: The Negative Control Lentivirus (Firefly Luciferase) are replication incompetent, HIV-based, VSV-G pseudo typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.

Negative Control eGFP Reporter Lentivirus

79927 500 µl x 2
EUR 835
Description: The Negative Control eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced Green Fluorescent Protein (eGFP) gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.

Expression Negative Control Lentivirus (G418)

79902-G 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Expression Negative Control Lentivirus (Puromycin)

79902-P 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Expression Negative Control Lentivirus (Hygromycin)

79902-H 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

AR-RFP (GFP) Lentivirus 

LVP913-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-Luc (GFP) Lentivirus 

LVP914-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-RFP (GFP) Lentivirus 

LVP950-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-Luc (GFP) Lentivirus 

LVP951-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

H_RE-RFP (GFP) Lentivirus 

LVP970-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

H_RE-Luc (GFP) Lentivirus 

LVP971-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ER-RFP (GFP) Lentivirus 

LVP974-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ER-Luc (GFP) Lentivirus 

LVP975-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-RFP (GFP) Lentivirus 

LVP1022-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Luc (GFP) Lentivirus 

LVP1023-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-Rluc (GFP) Lentivirus 

LVP915-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-RFP (GFP) Lentivirus 

LVP946-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-Luc (GFP) Lentivirus 

LVP947-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-Rluc (GFP) Lentivirus 

LVP952-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-RFP (GFP) Lentivirus 

LVP954-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-Luc (GFP) Lentivirus 

LVP955-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SRE-RFP (GFP) Lentivirus 

LVP958-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SRE-Luc (GFP) Lentivirus 

LVP959-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RBP_JK-RFP (GFP) Lentivirus 

LVP962-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RBP_JK-Luc (GFP) Lentivirus 

LVP963-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

H_RE-Rluc (GFP) Lentivirus 

LVP972-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ER-Rluc (GFP) Lentivirus 

LVP976-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

P53-RFP (GFP) Lentivirus 

LVP978-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

P53-Luc (GFP) Lentivirus 

LVP979-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-RFP (GFP) Lentivirus 

LVP982-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (GFP) Lentivirus 

LVP983-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

B29-RFP (GFP) Lentivirus 

LVP994-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

B29-Luc (GFP) Lentivirus 

LVP995-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Rluc (GFP) Lentivirus 

LVP1024-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-RFP (GFP) Lentivirus 

LVP1026-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Luc (GFP) Lentivirus 

LVP1027-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-RFP (GFP) Lentivirus 

LVP1034-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Luc (GFP) Lentivirus 

LVP1035-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-RFP (GFP) Lentivirus 

LVP1042-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Luc (GFP) Lentivirus 

LVP1043-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-RFP (GFP) Lentivirus 

LVP1046-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-Luc (GFP) Lentivirus 

LVP1047-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-RFP (GFP) Lentivirus 

LVP938-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-Luc (GFP) Lentivirus 

LVP939-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-RFP (GFP) Lentivirus 

LVP942-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-Luc (GFP) Lentivirus 

LVP943-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-Rluc (GFP) Lentivirus 

LVP948-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-Rluc (GFP) Lentivirus 

LVP956-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SRE-Rluc (GFP) Lentivirus 

LVP960-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RBP_JK-Rluc (GFP) Lentivirus 

LVP964-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

NFkB-RFP (GFP) Lentivirus 

LVP966-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

NFkB-Luc (GFP) Lentivirus 

LVP967-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

P53-Rluc (GFP) Lentivirus 

LVP980-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (GFP) Lentivirus 

LVP984-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CEBP-RFP (GFP) Lentivirus 

LVP986-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CEBP-Luc (GFP) Lentivirus 

LVP987-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

B29-Rluc (GFP) Lentivirus 

LVP996-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD14-RFP (GFP) Lentivirus 

LVP998-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD14-Luc (GFP) Lentivirus 

LVP999-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-RFP (GFP) Lentivirus 

LVP1002-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Luc (GFP) Lentivirus 

LVP1003-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-RFP (GFP) Lentivirus 

LVP1006-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (GFP) Lentivirus 

LVP1007-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-RFP (GFP) Lentivirus 

LVP1010-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-Luc (GFP) Lentivirus 

LVP1011-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-RFP (GFP) Lentivirus 

LVP1014-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-Luc (GFP) Lentivirus 

LVP1015-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Rluc (GFP) Lentivirus 

LVP1028-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-RFP (GFP) Lentivirus 

LVP1030-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-Luc (GFP) Lentivirus 

LVP1031-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Rluc (GFP) Lentivirus 

LVP1036-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Rluc (GFP) Lentivirus 

LVP1044-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-Rluc (GFP) Lentivirus 

LVP1048-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-RFP (GFP) Lentivirus 

LVP1120-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-RFP (GFP) Lentivirus 

LVP1120-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in PBS solution.

EGR1-Luc (GFP) Lentivirus 

LVP1121-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-Luc (GFP) Lentivirus 

LVP1121-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in PBS solution.

GFAP-RFP (GFP) Lentivirus 

LVP1126-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-RFP (GFP) Lentivirus 

LVP1126-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-Luc (GFP) Lentivirus 

LVP1127-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-Luc (GFP) Lentivirus 

LVP1127-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.

ISRE-Rluc (GFP) Lentivirus 

LVP940-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-Rluc (GFP) Lentivirus 

LVP944-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

NFkB-Rluc (GFP) Lentivirus 

LVP968-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CEBP-Rluc (GFP) Lentivirus 

LVP988-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GATA2-RFP (GFP) Lentivirus 

LVP990-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under GATA-2 promoter which dynamically expressed in hematopoietic tissues and in the central nervous system. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GATA2-Luc (GFP) Lentivirus 

LVP991-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under GATA-2 promoter which dynamically expressed in hematopoietic tissues and in the central nervous system. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD14-Rluc (GFP) Lentivirus 

LVP1000-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

 

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

 

Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely replicate human biology and the pathophysiology of human illness. Right here we report a sturdy, reproducible, and scalable protocol for the technology of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing quick publicity to nonengineered matrices.

These hepatic organoids observe outlined levels of hepatic growth and categorical increased ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to a lot of metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.

We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs beneath each 2D tradition and three-dimensional (3D) organoid circumstances. Beneath typical 2D tradition circumstances, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In distinction, when cultured as 3D hepatic organoids, the differentiation defects have been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the useful interrogation of genes recognized by means of human genetic investigation.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF7 [MCF-7] Cell Line

CL-0149 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

PKCa Stable Expressing MCF-7 (MCF-7/PKCa20) Cell Line

T6115 1x10^6 cells / 1.0 ml
EUR 3950

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

MCF 10A Cell Line

CL-0525 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

cDNA - Human Tumor Cell Line: MCF 7

C1255830 40 reactions
EUR 385

Flavopiridol-Resistant MCF-7 Cell Line (FLV100)

T8033 1x10^6 cells / 1.0 ml
EUR 2950

Flavopiridol-Resistant MCF-7 Cell Line (FLV1000)

T8035 1x10^6 cells / 1.0 ml Ask for price

Total RNA - Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 191

Genomic DNA - Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 245

Total Protein - Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 188

Membrane Protein - Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 270

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 238

Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line

T3161 1x10^6 cells / 1.0 ml
EUR 3950

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 582
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 490.8
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Radius 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 602.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 2362.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 622.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 2454
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 637.2
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 3463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 24-well Cell Invasion, Fluorometric

CBA-111 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-well Cell Invasion, Fluorometric

CBA-112 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.

CytoSelect Cell Viability and Cytotoxicity Assay

CBA-240 96 assays
EUR 470.4
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

MCF2L2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence-like 2 (MCF2L2)

RG212640 10 µg Ask for price

CytoSelect 24-Well Cell Co-Culture System

CBA-160 24 assays
EUR 525.6
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

CytoSelect 24-Well Cell Co-Culture System

CBA-160-5 5 x 24 assays
EUR 2078.4
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

CytoSelect 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 762
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CytoSelect 24-well Cell Invasion Assay, Colorimetric

CBA-110 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 24-well Laminin Cell Invasion, Colorimetric

CBA-110-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 24-well Collagen Cell Invasion, Colorimetric

CBA-110-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

Radius 24-Well Cell Migration Assay, (Laminin Coated)

CBA-125-LN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

StemTAG PCR Primer Set for Stem Cell Characterization

CBA-303 1 kit
EUR 470.4
Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 96 assays
EUR 762
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Radius 24-Well Cell Migration Assay, (Fibronectin Coated)

CBA-125-FN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 409

Radius 24-Well Cell Migration Assay, (ECM Array Coated)

CBA-125-ECM 24 wells
EUR 838.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit

CBA-254 96 assays
EUR 672
Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts.  The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. 

CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetric)

CBA-056 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric)

CBA-057 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 24-well Laminin Cell Invasion Assay, Fluorometric

CBA-111-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 24-well Collagen Cell Invasion Assay, Fluorometric

CBA-111-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

Radius 24-Well Cell Migration Assay, (Collagen I Coated)

CBA-125-COL 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Colorimetric)

CBA-058 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Fluorometric)

CBA-059 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Colorimetric)

CBA-050 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibronectin.

CytoSelect 48-well Cell Adhesion Assay (Fibronectin, Fluorometric)

CBA-051 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibronectin.

CytoSelect 24-well Cell Migration Assay (8 ?m), Colorimetric

CBA-100 12 assays
EUR 651.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (8 ?m), Colorimetric

CBA-100-5 5 x 12 assays
EUR 2745.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (8 ?m), Fluorometric

CBA-101 12 assays
EUR 664.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (8 ?m), Fluorometric

CBA-101-5 5 x 12 assays
EUR 2787.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (5 ?m), Fluorometric

CBA-102 12 assays
EUR 664.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 24-well Cell Migration Assay (5 ?m), Fluorometric

CBA-102-5 5 x 12 assays
EUR 2787.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 24-well Cell Migration Assay (3 ?m), Fluorometric

CBA-103 12 assays
EUR 664.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 24-well Cell Migration Assay (3 ?m), Fluorometric

CBA-103-5 5 x 12 assays
EUR 2787.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric

CBA-104 96 assays
EUR 762
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (3 ?m), Fluorometric

CBA-104-5 5 x 96 assays
EUR 3135.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric

CBA-105 96 assays
EUR 762
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (5 ?m), Fluorometric

CBA-105-5 5 x 96 assays
EUR 3135.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 5 µm pore size is ideal for monocytes / macrophages.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106 96 assays
EUR 762
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 96-well Cell Migration Assay (8 ?m), Fluorometric

CBA-106-5 5 x 96 assays
EUR 3135.6
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

CytoSelect 24-well Cell Migration Assay (12 ?m), Colorimetric

CBA-107 12 assays
EUR 664.8
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their enviroment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 12 µm pore size is suitable for astrocytes and other large or slow-moving cells.

CytoSelect 24-well Cell Migration Assay (12 ?m), Fluorometric

CBA-108 12 assays
EUR 679.2
Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their enviroment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. The 12 µm pore size is suitable for astrocytes and other large or slow-moving cells.

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 1

RG212933 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 2

RG218217 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 4

RG230516 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5

RG230549 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 6

RG230587 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 3

RG230610 10 µg Ask for price

CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric)

CBA-070 48 assays
EUR 699.6
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control.

CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric)

CBA-070-5 5 x 48 assays
EUR 2836.8
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control.

CytoSelect 48-well Cell Adhesion Assay (ECM Array, Fluorometric)

CBA-071 48 assays
EUR 741.6
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control.

CytoSelect 48-well Cell Adhesion Assay (ECM Array, Fluorometric)

CBA-071-5 5 x 48 assays
EUR 3045.6
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control.

CytoSelect 48-well Cell Adhesion Assay (Collagen I, Colorimetric)

CBA-052 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Collagen I.

CytoSelect 48-well Cell Adhesion Assay (Collagen I, Fluorometric)

CBA-053 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Collagen I.

CytoSelect 48-well Cell Adhesion Assay (Collagen IV, Colorimetric)

CBA-060 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Collagen IV.

CytoSelect 48-well Cell Adhesion Assay (Collagen IV, Fluorometric)

CBA-061 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Collagen IV.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)

CBA-155-5 25 preps
EUR 3894
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

GFP ELISA Kit

AKR-121 96 assays
EUR 880.8
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

GFP ELISA Kit

AKR-121-5 5 x 96 assays
EUR 3630
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

GFP Quantitation Kit

AKR-120 100 assays
EUR 692.4
Description: Cell Biolabs? GFP Quantitation Kit measures GFP fluorescence in a fluorometer. The quantity of rGFP in sample is determined by comparing its fluorescence reading with that of known recombinant GFP standard curve. The kit has detection sensitivity limit of 100 ng/mL. A proprietary GFP quench solution is also included for determining autofluorescence of cell or tissue sample. Each kit provides sufficient reagents to perform up to 100 assays including standard curve and GFP samples.

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