Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

 

Background: Propofol is a standard sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been absolutely elucidated.

 

Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Equipment-8 (CCK-8) to detect proliferation. Apoptosis was measured with move cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or detrimental management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 

small interfering ribonucleic acid (siRNA) or detrimental management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression have been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR). In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice have been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors have been then collected from mice and analyzed by immunohistochemistry and Western blotting.

 

Outcomes: Propofol inhibited development (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by reducing PTEN protein

 

expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol remedy lowered NSCLC tumor development (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

 

Conclusions: Our examine indicated that propofol inhibited A549 cell development, accelerated apoptosis through the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell development, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical remedy.

 

Expression Negative Control Lentivirus (Puromycin)
79902-P	BPS Bioscience	500 µl x 2	EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Negative Control eGFP Reporter Lentivirus
79927	BPS Bioscience	500 µl x 2	EUR 835
Description: The Negative Control eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced Green Fluorescent Protein (eGFP) gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.

GFP Expressing Human Glioblastoma Cells
TR01-GFP	Neuromics	500,000 Cells	EUR 1624.8

GFP Expressing Human Gastric Carcinoma N87 Cells
TR02-GFP	Neuromics	500,000 Cells	EUR 1624.8

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)
TR04-GFP	Neuromics	500,000 Cells	EUR 1624.8

GFP Expressing Human Prostate Carcinoma Cells (DU 145)
TR03-GFP	Neuromics	500,000 Cells	EUR 1624.8

Lenti-HOXA10-GFP Lentivirus
LV646	ABM	10 ml	EUR 973.2

Lenti-HOXB8-GFP Lentivirus
LV634	ABM	10 ml	EUR 973.2

CD14-Rluc (GFP) Lentivirus
LVP1000-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (Bsd) Lentivirus
LVP1001-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (Neo) Lentivirus
LVP1001-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (Puro) Lentivirus
LVP1001-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (RFP) Lentivirus
LVP1001-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-RFP (GFP) Lentivirus
LVP1002-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Luc (GFP) Lentivirus
LVP1003-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Rluc (GFP) Lentivirus
LVP1004-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (Bsd) Lentivirus
LVP1005-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (Neo) Lentivirus
LVP1005-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (Puro) Lentivirus
LVP1005-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (RFP) Lentivirus
LVP1005-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-RFP (GFP) Lentivirus
LVP1006-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (GFP) Lentivirus
LVP1007-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Rluc (GFP) Lentivirus
LVP1008-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (Bsd) Lentivirus
LVP1009-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (Neo) Lentivirus
LVP1009-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (Puro) Lentivirus
LVP1009-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (RFP) Lentivirus
LVP1009-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-RFP (GFP) Lentivirus
LVP1010-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-Luc (GFP) Lentivirus
LVP1011-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-Rluc (GFP) Lentivirus
LVP1012-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (Bsd) Lentivirus
LVP1013-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (Neo) Lentivirus
LVP1013-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (Puro) Lentivirus
LVP1013-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (RFP) Lentivirus
LVP1013-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-RFP (GFP) Lentivirus
LVP1014-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-Luc (GFP) Lentivirus
LVP1015-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-Rluc (GFP) Lentivirus
LVP1016-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (Bsd) Lentivirus
LVP1017-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (Neo) Lentivirus
LVP1017-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (Puro) Lentivirus
LVP1017-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (RFP) Lentivirus
LVP1017-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-RFP (GFP) Lentivirus
LVP1018-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-Luc (GFP) Lentivirus
LVP1019-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-Rluc (GFP) Lentivirus
LVP1020-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (Bsd) Lentivirus
LVP1021-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (Neo) Lentivirus
LVP1021-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (Puro) Lentivirus
LVP1021-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (RFP) Lentivirus
LVP1021-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-RFP (GFP) Lentivirus
LVP1022-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Luc (GFP) Lentivirus
LVP1023-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Rluc (GFP) Lentivirus
LVP1024-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (Bsd) Lentivirus
LVP1025-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (Neo) Lentivirus
LVP1025-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (Puro) Lentivirus
LVP1025-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (RFP) Lentivirus
LVP1025-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-RFP (GFP) Lentivirus
LVP1026-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Luc (GFP) Lentivirus
LVP1027-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Rluc (GFP) Lentivirus
LVP1028-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (Bsd) Lentivirus
LVP1029-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (Neo) Lentivirus
LVP1029-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (Puro) Lentivirus
LVP1029-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (RFP) Lentivirus
LVP1029-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-RFP (GFP) Lentivirus
LVP1030-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-Luc (GFP) Lentivirus
LVP1031-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-Rluc (GFP) Lentivirus
LVP1032-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (Bsd) Lentivirus
LVP1033-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (Neo) Lentivirus
LVP1033-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (Puro) Lentivirus
LVP1033-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (RFP) Lentivirus
LVP1033-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-RFP (GFP) Lentivirus
LVP1034-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Luc (GFP) Lentivirus
LVP1035-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Rluc (GFP) Lentivirus
LVP1036-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (Bsd) Lentivirus
LVP1037-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (Neo) Lentivirus
LVP1037-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (Puro) Lentivirus
LVP1037-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (RFP) Lentivirus
LVP1037-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-RFP (GFP) Lentivirus
LVP1038-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-Luc (GFP) Lentivirus
LVP1039-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-Rluc (GFP) Lentivirus
LVP1040-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (Bsd) Lentivirus
LVP1041-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (Neo) Lentivirus
LVP1041-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (Puro) Lentivirus
LVP1041-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (RFP) Lentivirus
LVP1041-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-RFP (GFP) Lentivirus
LVP1042-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Luc (GFP) Lentivirus
LVP1043-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Rluc (GFP) Lentivirus
LVP1044-G	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-GFP (Bsd) Lentivirus
LVP1045-B	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-GFP (Neo) Lentivirus
LVP1045-N	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-GFP (Puro) Lentivirus
LVP1045-P	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-GFP (RFP) Lentivirus
LVP1045-R	GenTarget	1x107 IFU/ml x 200ul	EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

 

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

 

Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely replicate human biology and the pathophysiology of human illness. Right here we report a sturdy, reproducible, and scalable protocol for the technology of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing quick publicity to nonengineered matrices.

These hepatic organoids observe outlined levels of hepatic growth and categorical increased ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to a lot of metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.

We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs beneath each 2D tradition and three-dimensional (3D) organoid circumstances. Beneath typical 2D tradition circumstances, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In distinction, when cultured as 3D hepatic organoids, the differentiation defects have been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the useful interrogation of genes recognized by means of human genetic investigation.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7
T2255830	Biochain	5 slides	EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total RNA from Human Tumor Cell Line: MCF 7
R1255830-50	Biochain	50 ug	EUR 232.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

MCF-7 Nuclear Extract (H2O2)
1642-100	Biovision	each	EUR 248.4

Human MCF-7 (breast cancer) Cell Nuclear Extract
HCL-2016	Alpha Diagnostics	100ug	EUR 255.6

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)
MCF7-100	Alpha Diagnostics	100 ug	EUR 196.8

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)
MCF7-50	Alpha Diagnostics	50 ug	EUR 153.6

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes
EXOP-100A-1	SBI	50 ug	EUR 560.4

293/GFP Cell Line
AKR-200	Cell Biolabs	1 vial	EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line
AKR-208	Cell Biolabs	1 vial	EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line
AKR-209	Cell Biolabs	1 vial	EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line
AKR-213	Cell Biolabs	1 vial	EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line
AKR-214	Cell Biolabs	1 vial	EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

MCF-10A
C0006015	Addexbio	One Frozen vial	EUR 560.4

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated
HCL-2014	Alpha Diagnostics	100ug	EUR 255.6

GFP Expressing Human Glioblastoma Cells
TR01-GFP	Neuromics	500,000 Cells	EUR 1624.8

GFP Expressing Human Gastric Carcinoma N87 Cells
TR02-GFP	Neuromics	500,000 Cells	EUR 1624.8

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)
TR04-GFP	Neuromics	500,000 Cells	EUR 1624.8

GFP Expressing Human Prostate Carcinoma Cells (DU 145)
TR03-GFP	Neuromics	500,000 Cells	EUR 1624.8

293AAV Cell Line
AAV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line
AD-100	Cell Biolabs	1 vial	EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293LTV Cell Line
LTV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line
RV-100	Cell Biolabs	1 vial	EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

NIH3T3/Cas9 Cell Line
AKR-5104	Cell Biolabs	1 vial	EUR 686.4

293/Cas9 Cell Line
AKR-5110	Cell Biolabs	1 vial	EUR 686.4

HeLa/Cas9 Cell Line
AKR-5111	Cell Biolabs	1 vial	EUR 686.4

IGHD1-7 3'UTR GFP Stable Cell Line
TU060550	ABM	1.0 ml	EUR 2799.6

IGHV3-7 3'UTR GFP Stable Cell Line
TU060641	ABM	1.0 ml	EUR 2799.6

IGHV3OR15-7 3'UTR GFP Stable Cell Line
TU060691	ABM	1.0 ml	EUR 2799.6

IGHV3OR16-7 3'UTR GFP Stable Cell Line
TU060693	ABM	1.0 ml	EUR 2799.6

IGKV2OR2-7 3'UTR GFP Stable Cell Line
TU060855	ABM	1.0 ml	EUR 2799.6

IGKV3-7 3'UTR GFP Stable Cell Line
TU060861	ABM	1.0 ml	EUR 2799.6

IGKV3D-7 3'UTR GFP Stable Cell Line
TU060868	ABM	1.0 ml	EUR 2799.6

IGLV3-7 3'UTR GFP Stable Cell Line
TU060934	ABM	1.0 ml	EUR 2799.6

KRTAP4-7 3'UTR GFP Stable Cell Line
TU062120	ABM	1.0 ml	EUR 1672.8

KRTAP5-7 3'UTR GFP Stable Cell Line
TU062132	ABM	1.0 ml	EUR 1672.8

KRTAP9-7 3'UTR GFP Stable Cell Line
TU062151	ABM	1.0 ml	EUR 1672.8

KRTAP10-7 3'UTR GFP Stable Cell Line
TU062161	ABM	1.0 ml	EUR 1672.8

KRTAP19-7 3'UTR GFP Stable Cell Line
TU062187	ABM	1.0 ml	EUR 1672.8

Krtap4-7 3'UTR GFP Stable Cell Line
TU256900	ABM	1.0 ml	Ask for price

RNU6-7 3'UTR GFP Stable Cell Line
TU070174	ABM	1.0 ml	EUR 2799.6

TRAV8-7 3'UTR GFP Stable Cell Line
TU076322	ABM	1.0 ml	EUR 2799.6

TRBJ2-7 3'UTR GFP Stable Cell Line
TU076380	ABM	1.0 ml	EUR 2799.6

TRBV5-7 3'UTR GFP Stable Cell Line
TU076394	ABM	1.0 ml	EUR 2799.6

TRBV6-7 3'UTR GFP Stable Cell Line
TU076402	ABM	1.0 ml	EUR 2799.6

TRBV7-7 3'UTR GFP Stable Cell Line
TU076411	ABM	1.0 ml	EUR 2799.6

Krtap16-7 3'UTR GFP Stable Cell Line
TU160815	ABM	1.0 ml	Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line
TU160834	ABM	1.0 ml	Ask for price

Anti-Cytokeratin 7
DB051RTU-7	DB Biotech	7 ml	EUR 244.8
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

SKOV-3/Luc Cell Line
AKR-232	Cell Biolabs	1 vial	EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line
AKR-254	Cell Biolabs	1 vial	EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Recombinant Human Galectin-7
7-00442	CHI Scientific	2µg	Ask for price

Recombinant Human Galectin-7
7-00443	CHI Scientific	10µg	Ask for price

Recombinant Human Galectin-7
7-00444	CHI Scientific	1mg	Ask for price

Recombinant Human Interleukin-7
7-00895	CHI Scientific	2µg	Ask for price

Recombinant Human Interleukin-7
7-00896	CHI Scientific	10µg	Ask for price

Recombinant Human Interleukin-7
7-00897	CHI Scientific	1mg	Ask for price

Recombinant Mouse Interleukin-7
7-00904	CHI Scientific	2µg	Ask for price

Recombinant Mouse Interleukin-7
7-00905	CHI Scientific	10µg	Ask for price

Recombinant Mouse Interleukin-7
7-00906	CHI Scientific	1mg	Ask for price

Recombinant Human Kallikrein-7
7-03034	CHI Scientific	2µg	Ask for price

Recombinant Human Kallikrein-7
7-03035	CHI Scientific	10µg	Ask for price

Recombinant Human Kallikrein-7
7-03036	CHI Scientific	100µg	Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03478	CHI Scientific	5µg	Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03479	CHI Scientific	20µg	Ask for price

Recombinant ProMatrix Metalloproteinase-7
7-03480	CHI Scientific	1mg	Ask for price

Individual Reaction Mix 7
G065-7	ABM	200 reactions	EUR 200.4

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative
800-420-GFP	Alpha Diagnostics	1 kit	EUR 854.4

Platinum-E Retroviral Packaging Cell Line, Ecotropic
RV-101	Cell Biolabs	1 vial	EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic
RV-102	Cell Biolabs	1 vial	EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic
RV-103	Cell Biolabs	1 vial	EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Recombinant Human Interleukin-7, Saccharomyces
7-00898	CHI Scientific	2µg	Ask for price

Recombinant Human Interleukin-7, Saccharomyces
7-00899	CHI Scientific	10µg	Ask for price

Recombinant Human Interleukin-7, Saccharomyces
7-00900	CHI Scientific	1mg	Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03154	CHI Scientific	5µg	Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03155	CHI Scientific	20µg	Ask for price

Recombinant Human Matrix Metalloproteinase-7
7-03156	CHI Scientific	1mg	Ask for price

Lung Lysate (7 Days Old)
1402-7	ProSci	0.1 mg	EUR 229.2
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)
1403-7	ProSci	0.1 mg	EUR 229.2
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)
1404-7	ProSci	0.1 mg	EUR 229.2
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)
1405-7	ProSci	0.1 mg	EUR 229.2
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)
1406-7	ProSci	0.1 mg	EUR 229.2
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)
1409-7	ProSci	0.1 mg	EUR 229.2
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)
1415-7	ProSci	0.1 mg	EUR 229.2
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)
1419-7	ProSci	0.1 mg	EUR 229.2
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)
1420-7	ProSci	0.1 mg	EUR 229.2
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

ICP Quality Control Standard 7
QC-7	Scientific Laboratory Supplies	125ML	EUR 176.7

Total Protein - Murine Embryonic Stem Cell Line D3
CBA-305	Cell Biolabs	500 ?g	EUR 414
Description: Isolated from mouse ES-D3 cell line Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Recombinant Human Bone Morphogenetic protein-7
7-00184	CHI Scientific	2µg	Ask for price

Recombinant Human Bone Morphogenetic protein-7
7-00185	CHI Scientific	10µg	Ask for price

Recombinant Human Bone Morphogenetic protein-7
7-00186	CHI Scientific	1mg	Ask for price

Recombinant Human Galectin-7 His Tag
7-00445	CHI Scientific	5µg	Ask for price

Recombinant Human Galectin-7 His Tag
7-00446	CHI Scientific	20µg	Ask for price

Recombinant Human Galectin-7 His Tag
7-00447	CHI Scientific	1mg	Ask for price

Recombinant Human Interleukin-7, His Tag
7-00901	CHI Scientific	2µg	Ask for price

Recombinant Human Interleukin-7, His Tag
7-00902	CHI Scientific	10µg	Ask for price

Recombinant Human Interleukin-7, His Tag
7-00903	CHI Scientific	1mg	Ask for price

Recombinant Human Retinol Binding Protein-7
7-01543	CHI Scientific	5µg	Ask for price

Recombinant Human Retinol Binding Protein-7
7-01544	CHI Scientific	20µg	Ask for price

Recombinant Human Retinol Binding Protein-7
7-01545	CHI Scientific	1mg	Ask for price

Recombinant Human Parathyroid Hormone (7-34)
7-02317	CHI Scientific	100µg	Ask for price

Recombinant Human Parathyroid Hormone (7-34)
7-02318	CHI Scientific	500µg	Ask for price

Recombinant Human Parathyroid Hormone (7-34)
7-02319	CHI Scientific	1mg	Ask for price

Recombinant Human Calcium Binding Protein 7
7-04639	CHI Scientific	1µg	Ask for price

Recombinant Human Calcium Binding Protein 7
7-04640	CHI Scientific	5µg	Ask for price

Recombinant Human Calcium Binding Protein 7
7-04641	CHI Scientific	50µg	Ask for price

Recombinant Human Nuclear Import 7 Homolog
7-05707	CHI Scientific	5µg	Ask for price

Recombinant Human Nuclear Import 7 Homolog
7-05708	CHI Scientific	25µg	Ask for price

Recombinant Human Nuclear Import 7 Homolog
7-05709	CHI Scientific	1mg	Ask for price

Recombinant Human Parkinson Disease Protein 7
7-05800	CHI Scientific	5µg	Ask for price

Recombinant Human Parkinson Disease Protein 7
7-05801	CHI Scientific	20µg	Ask for price